HOME研究室紹介食品微生物学研究室

研究室紹介

食品微生物研究室

専門分野

応用微生物学・構造生物学

教員名・職位

村田 幸作教授

個人DB:under construction

個人HP:under construction

村田 幸作 教授

研究内容

応用微生物学、分子生物学、構造生物学、網羅的解析学など、生命系多分野の先端バイオテクノロジーを用いた微生物、就中、食品関連微生物の解析とその食品・医薬・化学・環境への応用に関する研究。特に、巨大分子(多糖、DNA、人工高分子ポリマー)の輸送と分解の構造生物学・進化生物学および新規酵素の創成生物学、細菌の表層構造と運動制御、生体エネルギー担体の化学進化とNADキナーゼの分子生物学、遺伝子組換え作物・食品の作成と安全性、海/空圏バイオテクノロジーによるエネルギー・有用物質の生産。
業績
学術論文
  1. Yukie Maruyama, Takafumi Itoh, Ai Kaneko, Yu Nishitani, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of bacterial ABC transporter involved in import of acidic polysaccharide alginate. submitted (2014).
  2. Wataru Hashimoto, Yukie Maruyama, Yusuke Nakamichi, Bunzo Mikami, and Kousaku Murata: Crystal structure of Pedobacter heparinus Heparin Lyase Hep III with the acive site in a deep cleft. Biochemistry, 53(4), 777-786 (2014).
  3. Yusuke Nakamichi, Bunzo Mikami, Kousaku Murata, and Wataru Hashimoto: Crystal structure of a bacterial unsaturated glucuronyl hydrolase with specificity for heparin. J.Biol.Chem., 289(8), 4787-4797 (2014).
  4. Yusuke Nakamichi, Aya Yoshioka, Shigeyuki Kawai, and Kousaku Murata: Conferring the ability to utilize inorganic polyphosphate on ATP-specific NAD kinase. Sci. Rep., 3, 2632;DOI:10.1038/srep02632 (2013).  
  5. Kazuto Ohashi, Shigeyuki Kawai, and Kousaku Murata: Secretion of quinolinic acid to reutilize for NAD+ biosynthesis in yeast Saccharomyces cerevisiae. Eukaryot. Cell, 12(5):648-653 (2013). Global Medical Discovery (http://globalmedicaldiscovery.com)
  6. Kazuto Ohashi, Shigeyuki Kawai, and Kousaku Murata: Identification and characaterization of a human mitochondorial NAD kinase. Nat.Commun., 4, December 2012. DOI:10.1038/ncomms2262 (2012).
  7. Yu Nishitani, Yukie Maruyama, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Recognition of heteropolysaccharide alginate by periplasmic solute-binding proteins of a bacterial ABC transporter. Biochemistry, 51(17), 3622-3633 (2012).
  8. Bunzo Mikami, Mizuho Ban, Sachiko Suzuki, Hye-Jin Yoon, Osamu Miyake, Masayuki Yamasaki, Kohei Ogura, Yukie Maruyama, Wataru Hashimoto, and Kousaku Murata: Induced-fit motion of a lid loop involved in catalysis in alginate lyase A1-III. Acta Cryst., 68,1207-1216 (2012) .
  9. Yukie Maruyama, Asako Chuma, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Heterosubunit composition and crystal structures of a novel bacterial M16B metallopeptidase. J.Mol.Biol., 407(1), 180-192 (2011).
  10. Yukie Maruyama, Akihito Ochiai, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of bacterial cell-surface alginate-binding protein with an M_75 peptidase motif. Biochem.Biophys.Res.Commun., 405(3), 411-416 (2011).
  11. Yusuke Nakamichi, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Structural determinants in streptococcal unsaturated glucuronyl hydrolase for recognition of glycosaminoglycan sulfate groups. J.Biol.Chem., 286(8), 6262-6271 (2011).
  12. Hiroyuki Takeda, Fuminori Yoneyama, Shigeyuki Kawai, Wataru Hashimoto, and Kousaku Murata: Bioethanol production from marine biomass alginate by metabolically engineered bacteria. Energy Environ.Sci., 4, 2575-2581 (2011).
  13. Takuya Ando, Kazuto Ohashi, Akihito Ochiai, Bunzo Mikami, Shigeyuki Kawai, and Kousaku Murata: Structural determinants of discrimination of NAD+ from NADH in yeast mitochondrial NADH kinase Pos5. J.Biol.Chem., 286(34), 29984-29992 (2011).
  14. Akihito Ochiai, Masayuki Yamasaki, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of exotype alginate lyase Atu3025 from Agrobacterium tumefaciens. J.Biol.Chem., 285(32), 24519-24528 (2010).
  15. Wataru Hashimoto, Akihito Ochiai, Keiko Momma, Takafumi Itoh, Bunzo Mikami, Yukie Maruyama, and Kousaku Murata: Crystal structure of the glycosidase family 73 peptidoglycan hydrolase FlgJ. Biochem.Biophys.Res.Commun., 381(1), 16-21 (2009).
  16. Hikaru Miyagi, Shigeyuki Kawai, and Kousaku Murata: Two sources of mitochondrial NADPH in the yeast Saccharomyces cerevisiae. J.Biol.Chem., 284(12), 7553-7560 (2009).
  17. Akihito Ochiai, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Structural determinants responsible for substrate recognition and mode of action in family 11 polysaccharide lyases. J.Biol.Chem., 284(15), 10181-10189 (2009).
  18. Yukie Maruyama, Yusuke Nakamichi, Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Substrate specificity of Streptococcal unsaturated glucuronyl hydrolase for sulfated glycosaminoglycan. J.Biol.Chem., 284(27), 18059-18069 (2009).
  19. Kohei Ogura, Masayuki Yamasaki, Takashi Yamada, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of family 14 polysaccharide lyase with pH-dependent modes of action. J.Biol.Chem., 284 (51), 35572-35579 (2009).
  20. Takafumi Itoh, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of YihS in complex with D-mannose: Structural annotation of Escherichia coli and Salmonella enterica yihS-encoded proteins to an aldose-ketose isomerase. J.Mol.Biol., 46(3), 781-791 (2008).
  21. Yukie Maruyama, Misato Momma, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of a novel bacterial cell-surface flagellin binding to polysaccharide. Biochemstry, 47(5), 1393-1402 (2008).
  22. Kohei Ogura, Masayuki Yamasaki, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Substrate recognitin by family 7 alginate lyase from Sphingomonas sp. A1. J.Mol.Biol., 380(2), 373-385 (2008).
  23. Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan. Biochemistry, 46(3), 781-791 (2007).
  24. Akihito Ochiai, Takafumi Itoh, Yukie Maruyama, Akiko Kawamata, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonyl lyases YesW with an eight-bladed β-propeller. J.Biol.Chem., 282(51), 37134-37145 (2007).
  25. Zhongli Cui, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of glycoside hydrolase family 78 α-L-rhamnosidase from Bacillus sp. GL1. J.Mol.Biol., 374(2), 384-398 (2007).
  26. Takafumi Itoh, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1. Biochem.Bophys.Res.Commun., 344(1), 253-262 (2006).
  27. Yuji Aso, Yukiko Miyamoto, Karen Mine Harada, Keiko Momma, Shigeyuki Kawai, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria. Nat.Biotechnol., 24(2), 188-189(2006).
  28. Takafumi Itoh, Akihito Ochiai, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: A novel glycoside hydrolase Family 105: The structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide. J.Mol.Biol., 360(3), 573-585 (2006).
  29. Takafumi Itoh, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Crystal structure of unsaturated glucuronyl hydrolases complexed with substrate. J.Biol.Chem., 281(40), 29807-29816 (2006).
  30. Masayuki Yamasaki, Kohei Ogura, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: A structural basis for depolymerization of alginate by polysaccharide lyase family-7. J.Mol.Biol., 352(1), 11-21 (2005).
  31. Yukie Maruyama, Wataru Hashimoto, Bunzo Mikami and Kousaku Murata: Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism. J.Mol.Biol., 350(5), 974-986 (2005).
  32. Keiko Momma, Yumiko Mishima, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Direct evidence for Sphingomonas sp. A1 periplasmic proteins as macromolecule-binding proteins associated with the ABC transporter: molecular insights into alginate transport in the periplasm. Biochemistry, 44(13), 5053-5064 (2005).
  33. Wataru Hashimoto, Jinshan He, Yushin Wada, Hirokazu Nankai, Bunzo Mikami, and Kousaku Murata: Proteomics-based identification of outer-membrane proteins responsible for import of macromolecules in Sphingomonas sp. A1: alginate-binding flagellin on the cell surface. Biochemistry, 44(42), 13783-13794 (2005).
  34. Shigetarou Mori, Masayuki Yamasaki, Yukie Maruyama, Keiko Momma, Shigeyuki Kawai, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex.  Biochem.Biophys.Res.Commun., 327(2), 500-508 (2005).
  35. Shigeyuki Kawai, Chikako Fukuda, Takako Mukai, and Kousaku Murata: MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinase. J.Biol.Chem., 280(47), 39200-39207 (2005).
  36. Shigetarou Mori, Shigeyuki Kawai, Feng Shi, Bunzo Mikami, and Kousaku Murata: Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitution. J.Biol.Chem., 280(25), 24104-24112 (2005).
  37. Masayuki Yamasaki, Satoko Moriwaki, Osamu Miyake, Wataru Hashimoto, Kousaku Murata, and Bunzo Mikami: Structure and function of a hypothetical Pseudomonas aeruginosa protein PA1167 classified into family PL-7. J.Biol.Chem., 279(30), 31863-31872 (2004).
  38. Takafumi Itoh, Sae Akao, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Crystal structure of unsaturated glucuronyl hydrolase responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 Å resolution. J.Biol.Chem., 279(30), 31804-31812 (2004).
  39. Jinshan He, Hirokazu Nankai, Wataru Hashimoto, and Kousaku Murata: Molecular identification and characterization of an alginate-binding protein on the cell surface of Sphingomonas sp. A1. Biochem.Biophys.Res.Commun., 322(3), 712-717 (2004).
  40. Takako Mukai, Shigeyuki Kawai, Shigetarou Mori, and Kousaku Murata: Crystal structure of bacterial inorganic polyphosphate/ATP-glucomannokinase: INSIGHTS INTO KINASE EVOLUTION. J.Biol.Chem., 279(48), 50591-50600 (2004).
  41. Shigeyuki Kawai, Tuan Anh Pham, Ha Thu Nguyen, Hirokazu Nankai, Takaaki Utsumi, Yasuki Fukuda, and Kousaku Murata: Molecular insights on DNA delivery into Saccharomyces cerevisiae. Biochem.Biophys.Res.Commun., 317(1), 100-107 (2004).
  42. Yumiko Mishima, Keiko Momma, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1, complexed with an alginate tetrasaccharide at 1.6 Å resolution. J.Biol.Chem., 278(8), 6552-6559 (2003).
  43. Wataru Hashimoto, Hirokazu Nankai, Bunzo Mikami, and Kousaku Murata: Crystal structure of Bacillus sp. GL1 xanthan lyase, which acts on the side chains of xanthan. J.Biol.Chem., 278(9), 7663-7673 (2003).
  44. Keiko Momma, Bunzo Mikami, Yumiko Mishima, Wataru Hashimoto, and Kousaku Murata: Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0Å resolution. J.Mol.Biol., 316(5), 1061-1069 (2002).
  45. Yoshiyuki Hayama, Yasuki Fukuda, Shigeyuki Kawai, Wataru Hashimoto, and Kousaku Murata: Extremely simple, rapid, and highly efficient transformation method for the yeast Saccharomyces cerevisiae using glutathione and early log phase cells. J.Biosci.Bioeng., 94(2), 166-171 (2002).
  46. Hye-Jin Yoon, Wataru Hashimoto, Osamu Miyake, Kousaku Murata, and Bunzo Mikami: Crystal structure of alginate lyase A1-III complexed with trisaccharide product at 2.0 Å resolution. J.Mol.Biol., 307(1), 9-16 (2001).
  47. Shigeyuki Kawai, Shigetarou Mori, Takako Mukai, Hirokazu Matsukawa, Yushi Matuo, and Kousaku Murata: Establishment of a mass-production system for NADP using bacterial inorganic polyphosphate/ATP-NAD kinase. J.Biosci.Bioeng., 92(5), 47-452 (2001). 日本生物工学会論文賞・NADP生産工業化
  48. Takafumi Itoh, Bunzo Mikami, Isafumi Maru, Yasuhiro Ohta, Wataru Hashimoto, and Kousaku Murata: Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 Å resolution. J.Mol.Biol., 303(5), 733-744 (2000).
  49. Keiko Momma, Masako Okamoto, Yumiko Mishima, Shigetarou Mori, Wataru Hashimoto, and Kousaku Murata: A novel bacterial ATP-binding cassette transporter system that allows uptake of macromolecules. J.Bacteriol., 182(14), 3998-4004 (2000).
  50. Shigeyuki Kawai, Shigetarou Mori, Takako Mukai, Sachiko Suzuki, Takeshi Yamada, Wataru Hashimoto, and Kousaku Murata: Inorganic polyphosphate/ATP-NAD kinase of Micrococcus flavus and Mycobacterium tuberculosis H37Rv. Biochem.Biophys.Res.Commun., 276(1), 57-63 (2000).
  51. Hye-Jin Yoon, Bunzo Mikami, Wataru Hashimoto, and Kousaku Murata: Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78Å resolution. J.Mol.Biol., 290(2), 505-514 (1999).
  52. Isafumi Maru, Yasuhiro Ohta, Kousaku Murata, and Yoji Tsukada: Molecular cloning and identification of N-acyl-D-glucosamine 2-epimerase from porcine kidney as a renin-binding protein. J.Biol.Chem., 271(27), 16294-16299 (1996).
  53. Tomohiro Hisano, Nobuko Kimura, Wataru Hashimoto, and Kousaku Murata: Pit structure on bacterial cell surface. Biochem.Biophys.Res.Commun., 220(3):979-982 (1996).
  54. Tomohiro Hisano, Tomoko Inose, Tetsuo Yamashita, and Kousaku Murata: Direct uptake of alginate molecule through a pit on bacterial cell surface: a novel mechanism to take up macromolecules. J.Ferment.Bioeng., 79(6), 538-544 (1995). 日本生物工学会論文賞
  55. William R. Moore, Mary E. Anderson, Alton Meister, Kousaku Murata, and Akira Kimura: Increased capacity for glutathione synthesis enhances resistance to radiation in Escherichia coli: A possible model for mammalian cell protection. Proc.Natl.Acad.Sci.USA, 86(5), 1461-1464 (1989).
  56. Hisao Ito, Yasuki Fukuda, Kousaku Murata*, and Akira Kimura: Transformation of intact yeast cells treated with alkali cations. J.Bacteriol., 153(1), 163-168 (1983). *責任著者 被引用率約7000回、微生物分野で世界第2位。
  57. Saburo Komatsubara, Kousaku Murata, Masahiko Kisumi, and Ichiro Chibata: Threonine production by regulatory mutants of Serratia marcescens. Appl.Environ.Microbiol., 35(5), 834-840 (1978). スレオニン発酵工業化
  58. Saburo Komatsubara, Kousaku Murata, Masahiko Kisumi, and Ichiro Chibata: Threonine degradation by Serratia marcescens. J.Bacteriol., 135(2), 318-323 (1978).
他 省略
総説
  1. Shigeyuki Kawai, and Kousaku Murata: Transformation of intact cells of Saccharomyces cerevisiae: original and modified lithium methods, and possible underlying mechanism. Genetic Transformation Systems in Fungi  (Marco van den Berg, Karunakaran Maruthachalam eds.), Springer, in press (2014).
  2. Mitunori Yanagisawa, Shigeyuki Kawai, and Kousaku Murata: Strategies for the production of high concentration of bioethanol from seaweeds: Production of high concentration of bioethanol from seaweeds. Bioengineered, 4(4), 224-235 (2013).
  3. Wataru Hashimoto, Shigeyuki Kawai, and Kousaku Murata: Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications. Bioeng. Bugs, 1 (2), 97-109 (2010).
  4. Shigeyuki Kawai, Wataru Hashimoto, and Kousaku Murata: Transformation of Saccharomyces cerevisiae and other fungi: Methods and possible underlying mechanism. Bioeng. Bugs,, 1(6):395-403 (2010).
  5. Wataru Hashimoto, Yukie Maruyama, Takafumi Itoh, Bunzo Mikami, and Kousaku Murata: Bacterial system for alginate uptake and degradation. In "Alginate: Biology and Applications" (Ed. B.H.A. Rehm), Microbiology Monographs, 13, 73-94 (2009).
  6. Kousaku Murata, Shigeyuki Kawai, Bunzo Mikami, and Wataru Hashimoto: Superchannel of Bacteria: Biological Significance and New Horizons. Biosci.Biotechnol.Biochem., 72(2), 265-277 (2008). 日本農芸化学会賞受賞論文
  7. Shigeyuki Kawai, and Kousaku Murata: Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H). Biosci.Biotechnol.Biochem., 72(4),919-930 (2009).
  8. 河井重幸, 福田千加子, 村田幸作: NADP(H)合成の鍵酵素, NADキナーゼの機能と構造. 蛋白質・核酸・酵素, 152(3), 243-248 (2007).
  9. 橋本 渉, 丸山如江, 麻生祐司, 村田幸作: 細菌器官の分子移植と環境浄化. 化学と生物, 45(5), 320-329 (2007).
  10. Wataru Hashimoto, Takafumi Itoh, Yukie Maruyama, Bunzo Mikami, and Kousaku Murata: Hydration of vinyl ether groups by unsaturated glycoside hydrolases and their role in bacterial pathogenesis. International Microbiology, 10, 233-243 (2007).
  11. Shigeyuki Kawai, Takako Mukai, Shigetarou Mori, Bunzo Mikami, and Kousaku Murata: Hypothesis: structures, evolution, and ancestor of glucose kinases in the hexokinase family. J.Biosci.Bioeng., 99(4), 320-330 (2005).
  12. Wataru Hashimoto, Keiko Momma, Yukie Maruyama, Masayuki Yamasaki, Bunzo Mikami, and Kousaku Murata: Structure and function of bacterial super-biosystem responsible for import and depolymerization of macromolecules. Biosci.Biotechnol.Biochem., 69(4), 673-692 (2005).
  13. Wataru Hashimoto, Masayuki Yamasaki, Takafumi Itoh, Keiko Momma, Bunzo Mikami, and Kousaku Murata: Super-channel in bacteria: structural and functional aspects of a novel biosystem for the import and depolymerization of macromolecules. J.Biosci.Bioeng., 98(6), 399-413 (2004).
  14. Yumiko Mishima, Keiko Momma, Osamu Miyake, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Super-channel in bacteria: macromolecule uptake and depolymerization systems of Sphingomonas sp. A1 with special cell surface structure. Biotechnol.Genet.Eng.Rev., 19, 105-119 (2002).
  15. 橋本 渉, 三上文三, 村田幸作:細菌「超チャネル」の構造と高分子輸送機能. 生化学, 74(7), 563-567 (2002).
  16. Wataru Hashimoto, Keiko Momma, Yumiko Mishima, Bunzo Mikami, and Kousaku Murata: Super-channel in bacteria: Function and structure of a macromolecule import system mediated by a pit-dependent ABC transporter. Biosci.Biotechnol.Biochem., 65(9), 1949-1956 (2001).
  17. Yumiko Mishima, Keiko Momma, Wataru Hashimoto, Bunzo Mikami, and Kousaku Murata: Super-channel in bacteria: Function and structure of the macromolecule import system mediated by a pit-dependent ABC transporter. FEMS Microbiol.Lett., 204(2), 215-221 (2001).
  18. Wataru Hashimoto, Keiko Momma, Hikaru Miki, Yumiko Mishima, Eiko Kobayashi, Osamu Miyake, Shigeyuki Kawai, Hirokazu Nankai, Bunzo Mikami, and Kousaku Murata: Enzymatic and Genetic Bases on Assimilation, Depolymerization, and Transport of Heteropolysaccharides in Bacteria. J.Biosci.Bioeng., 87(2), 123-136 (1999).
  19. Keiko Momma, Wataru Hashimoto, Osamu Miyake, Hye-Jin Yoon, Shigeyuki Kawai, Yumiko Mishima, Bunzo Mikami, and Kousaku Murata: Special cell surface structure, and novel macromolecule transport/depolymerization system of Sphingomonas sp A1. J.Indust.Microbiol.Biotechnol., 23(4/5), 425-435 (1999).
  20. Kousaku Murata: Bacterial Alginate Lyase: Enzymatic and Genetic Aspects of the Lyase and Its Application to Agricultural, Food, and Pharmaceutical Processes. COMMENTS ON AGRIC. & FOOD CHEM., 3(2), 87-110 (1994).
  21. Kousaku Murata: Use of Microbial Spores as a Biocatalyst. CRITICAL REVIEW IN BIOTECHNOLOGY (Eds. G. G. Stewart and I. Russell), CRC Press, Inc., New York, 13(3), 173-193 (1993).
  22. Kousaku Murata, Tomoko Inose, Tomohiro Hisano, Shiro Abe, Yoshimasa Yonemoto, Tetsuo Yamashita, Masahiko Takagi, Kenji Sakaguchi, Akira Kimura, and Tadayuki Imanaka: Bacterial Alginate Lyase: Enzymology, Genetics and Application. J.Ferment.Bioeng., 76(5), 427-437 (1993).
他 省略
著書
  1. 「遺伝子から見た応用微生物学」(熊谷英彦, 加藤暢夫, 村田幸作, 阪井康能), 朝倉書店, 2008.
  2. 第6章 食品と微生物, pp.147-174,「生物資源から考える21世紀の農学」第6巻, 微生物機能の開発(植田充美 編), 京都大学出版会, 2008.
  3. 微生物細胞の構造・機能, -細菌の構造と機能- pp.74-88. 「応用微生物学」(第2版), 清水 昌, 堀ノ内末治 編, 文永堂出版, 2006.
  4. Wataru Hashimoto, Yumiko Mishima, Osamu Miyake, Hirokazu Nankai, Keiko Momma, and Kousaku Murata: Chapter 9, Biodegradation of alginate, xanthan, and gellan. In “Biopolymers” Vol.7: Polysaccharides I (Ed. A.Steinbuchel), pp.175-199, Wiley-VCH (2002).
  5. Keiko Momma, Wataru Hashimoto, Shigeru Utsumi, and Kousaku Murata: Safety assessment of genetically modified rice and potatoes with soybean glycinin. In “Testing for genetic manipulation in plants”, Molecular Methods of Plant Analysis, Vol.22 (Eds. J. F. Jackson (Managing Editor), H. F. Linslens, R.B. Inman), pp.139-152, Springer-Verlag Berlin Heidelberg (2002).
  6. 遺伝子組み換え食品がわかる本 (村田幸作, 清水 誠編), 法研出版, 2000.
  7. バイオテクノロジーと食品の安全性 ~第一世代の遺伝子組み換え食品と社会的受容~. pp.72-91,「21世紀に何を食べるか」, 恒星出版, 2000. 
  8. 遺伝子組み換え作物の近未来~どう安全を評価するか~, 市民のための「遺伝子問題」入門(奥野卓司・ヒューマンルネッサンス研究所 編), 岩波書店, 1994.
  9. Kousaku Murata: Glutathione and Its Derivatives: Produced by Recombinant Escherichia coli and Saccharomyces cerevisiae. RECOMBINANT MICROBES (Eds. Yoshikatsu Murooka and Tadayuki Imanaka), Marcel Dekkar Inc., New York, pp.159-183 (1994).
  10. 「遺伝子工学」 (木村 光, 村田幸作編), 応用化学講座12, 朝倉書店, 1990.
  11. Kousaku Murata and Akira Kimura: Overproduction of Glutathione and Its Derivatives by Genetically Engineered Microbial Cells. In: BIOTECHNOLOGY ADVANCES (Eds. M. Moo-Young, J. D. Bu Lock, C. L. Cooney, and B. R. Glick), Pergamon Press Inc., Oxford, 8, pp.59-96 (1990).
  12. Kousaku Murata: Chapter 6: Immobilization, Chemical and Industrial Application, and Biosynthetic Preparation of Glutathione and Related Compounds. In: GLUTATHIONE: CHEMICAL, BIOCHEMICAL AND MEDICAL ASPECTS, PART-A (Eds. David Dolphin, Rozanne Poulson, and Olga Avramovic), John Wiley & Sons, Inc., New York, pp.187-242 (1989).
他 省略
受賞
  1. 日本農芸化学会 大会トピックス賞(3回:平成25年3月, 平成24年3月, 平成23年3月)
  2. 日本農芸化学会賞 平成19年3月
  3. 日本生物工学会論文賞(2回:平成13年10月,平成8年10月)
  4. 永井記念財団 研究奨励賞 平成5年2月
  5. 日本農芸化学会 農芸化学奨励賞 昭和63年4月
他 省略
特許

 

海洋バイオマスからのエタノール生産
 特許第4845070号、平成23年10月21
 出願人/特許権者: 国立大学法人京都大学, 株式会社 マルハニチロホールディングス
 発明者: 村田幸作, 橋本 渉, 河井重幸, 織田浩司, 庵原啓司, 三上文三, 竹田浩之, 米山史紀, 落合秋人
 国際出願番号: PCT/JP2010/064383
 国際公開番号: WO2011/024858酵母の形質転換方法 
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 特許第4088251号、平成20229
 出願人/特許権者: オリエンタル酵母工業株式会社
 発明者: 河井重幸, 村田幸作, 松川寛和, 冨迫昭一, 安藤与志夫, 松尾雄志
 国際出願番号PCT/JP002/006692
 国際公開番号WO2003-004654
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 特許第3682530号, 平成17年6月3日  
 出願人/特許権者: 国立大学法人京都大学

 発明者: 村田幸作, 橋本 渉, 河井重幸, 葉山善幸, 福田泰樹

 

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